FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005688981

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005688981
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7688050
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG42350.014.894108145
ATCTCGT75250.011.754441145
CGTATGC51700.011.63955145
CTCGTAT63700.011.267937145
TCTCGTA68750.011.178455145
TCGTATG58000.010.875266145
CATCTCG98500.010.010397145
CACATTG250700.08.878359145
GTCACAT270800.08.861922145
ACATTGG229400.08.786179145
CGTCTGA559450.08.669888145
ACGTCTG600850.08.627572145
TCCAGTC373350.08.583309145
GCCGTCT53350.08.561596145
CATTGGC225300.08.559908145
CCAGTCA353150.08.49943145
CACGTCT649150.08.454724145
CAGTCAC347950.08.438919145
CTGAACT542050.08.373049145
TCTGAAC554800.08.350511145
Produced by FastQC (version 0.11.5)