FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005688982

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005688982
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7688050
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTCGG15400.024.009045145
CCGTATC46700.013.195549145
CGCCGTA47350.012.401963145
TGGTCGC81700.011.535773145
GTGGTCG94000.010.874685145
CGTATCA41050.010.773134145
GCCGTAT46900.010.666002145
CGGTGGT122800.010.508654145
GAGTGTA285050.09.995325145
TCTCGGT147350.09.84023145
GTCGCCG77250.09.290993145
AGTGTAG272400.09.128774145
GTAGATC289600.09.01217145
GATCTCG188050.08.944171145
GTAGGGA506700.08.928102145
GTGTAGA263450.08.916044145
TAGGGAA480300.08.905634145
GGTCGCC91300.08.893499145
AGAGTGT328050.08.88406145
TCGCCGT68500.08.784416145
Produced by FastQC (version 0.11.5)