FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005688986

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005688986
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5868786
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTCGG10050.023.803406145
GATCTCG92750.011.489329145
TAGGGAA315150.011.087177145
CGTGTAG394000.010.910627145
GAGTGTA165650.010.853042145
GTGTAGG376350.010.805931145
AGTGTAG154750.010.72744145
TGTAGGG354650.010.710816145
GTGTAGA153850.010.5546145
GTAGATC166050.010.5213145
AGAGTGT195300.010.356028145
TCGTGTA419300.010.148562145
GTCGCCG36600.010.101372145
GTCGTGT447850.010.051949145
AGATCTC119800.09.984318145
CGTCGTG488750.09.922717145
TGTAGAT168350.09.860833145
GTAGGGA333200.09.8121195145
TCTCGGT79850.09.80483145
AGGGAAA348300.09.678114145
Produced by FastQC (version 0.11.5)