FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005688990

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005688990
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7331414
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTCGG12800.020.956585145
CGCCGTA35700.015.433845145
TGGTCGC65800.013.772501145
CGTATCA33450.012.353996145
CGGTGGT106700.012.298238145
GCCGTAT35950.012.099881145
GTGGTCG77850.011.454475145
TCTCGGT132900.011.292084145
TCGCCGT52550.011.036872145
CCGTATC35250.010.9004755145
GGTCGCC76250.010.173553145
GAGTGTA271000.010.11233145
AGTGTAG253550.09.864708145
GTAGATC257850.09.840783145
GTCGCCG59900.09.803631145
GATCTCG161800.09.723216145
TGTAGAT262750.09.629671145
AGATCTC195900.09.437012145
TAGATCT200850.09.384913145
GTGTAGA246700.09.34516145
Produced by FastQC (version 0.11.5)