FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005688992

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005688992
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6748974
Sequences flagged as poor quality0
Sequence length151
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTCGG13850.025.64956145
TGGTCGC44650.012.665046145
AGTGTAG195300.012.62148145
AGAGTGT247150.011.792315145
CGCCGTA21650.011.720436145
GCCGTAT22500.011.599883145
GAGTGTA211150.011.468035145
GTAGATC211200.011.122046145
GTGGTCG50600.011.032497145
GTGTAGA188400.010.967246145
TGTAGAT207700.010.960409145
TGTAGGG465850.010.81614145
TCTCGGT93000.010.602043145
GATCTCG120750.010.567182145
AGATCTC144550.010.532582145
TAGGGAA404850.010.314827145
TAGATCT146450.010.296926145
GTAGGGA435150.010.246363145
CGTGTAG510500.010.125754145
GGGAAAG399700.010.084962145
Produced by FastQC (version 0.11.5)