FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005688995

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005688995
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6773998
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTCAATGAATCTCGTAT103820.1532625194161557TruSeq Adapter, Index 1 (97% over 37bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG64950.012.166885145
CCTCAAT223850.010.687816145
GTCACCT315450.010.595037145
TCTCGTA75200.010.412092145
CACCTCA298950.010.209763145
TATGCCG50700.010.152722145
ATCTCGT78600.010.053934145
AGTCACC333900.010.03131145
CTCGTAT71100.09.686929145
CCAGTCA391750.09.623354145
CGTATGC58800.09.617217145
CTCAATG215600.09.549963145
TCACCTC313250.09.2808145
ACCTCAA276000.09.193716145
ATGAATC148650.09.1690645145
TCCAGTC421500.09.012926145
AACTCCA524700.08.912115145
CAATGAA211400.08.676561145
CTCCAGT464950.08.654039145
CAGTCAC383450.08.621607145
Produced by FastQC (version 0.11.5)