FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005688996

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005688996
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6773998
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG108660.16040748757233173Illumina Single End PCR Primer 1 (100% over 50bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTCGG13800.026.793587145
TCTCGGT161250.011.2403555145
GTGGTCG98900.011.142614145
GTGTAGA304450.011.073292145
GTAGATC328450.010.661484145
GATCTCG199050.010.635562145
TGGTCGC85450.010.60566145
AGTGTAG317200.010.491058145
AGATCTC232150.010.399568145
TAGATCT240200.010.383057145
TCGGTGG151750.010.080767145
TCGCCGT72900.010.044622145
CGGTGGT127300.010.023608145
GAGTGTA338100.09.778212145
AGAGTGT388550.09.758759145
CGCCGTA55350.09.692903145
GCCGTAT54150.09.639928145
TGTAGAT318650.09.191941145
GTCGCCG82100.09.18396145
CTCGGTG176700.09.067666145
Produced by FastQC (version 0.11.5)