FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005689003

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005689003
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6941450
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC28250.015.141678145
ATCTCGT47100.014.623224145
TCGTATG33350.013.478335145
CTCGTAT38500.013.181891145
TCTCGTA45500.013.066008145
CCTCAAT175600.011.725577145
TATGCCG22150.011.128731145
CTCAATG165550.010.860827145
AGTCACC268400.010.750805145
CCAGTCA323300.010.494955145
TCACCTC251950.010.4743595145
ACCTCAA223850.010.396528145
GTCACCT249550.010.255516145
GAACTCC481400.010.22596145
ACGTCTG637350.010.135382145
TCCAGTC348050.010.081941145
CAGTCAC317700.010.018154145
CACCTCA239250.09.697023145
CGTCTGA588400.09.6847925145
AACTCCA445600.09.566929145
Produced by FastQC (version 0.11.5)