FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005689004

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005689004
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6941450
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTCGG15600.022.30717145
CGCCGTA24950.016.56274145
GCCGTAT26550.014.472355145
TGGTCGC49150.013.570385145
GTGGTCG60450.013.432275145
TCTCGGT112300.013.23433145
CGGTGGT83150.012.380949145
GATCTCG140950.011.881596145
TAGATCT186450.011.820587145
GAGTGTA262900.011.747539145
AGATCTC173300.011.588016145
GTGTAGA234050.011.399008145
AGTGTAG245550.011.396599145
GTAGATC257450.011.179579145
AGAGTGT307800.011.023134145
TGTAGAT250500.010.621508145
TCGGTGG110650.010.2867145
GGGAAAG471650.010.16037145
GGTCGCC65850.010.128845145
TAGGGAA490900.010.116387145
Produced by FastQC (version 0.11.5)