FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005689012

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005689012
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7592293
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG359720.4737962562825223Illumina Single End PCR Primer 1 (100% over 50bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTCGG17750.033.49362145
TCCGATC8406.103821E-610.3565296
AGAGCGG41300.010.00625145
TTCCGAT12005.2573705E-79.0619635
TCGCGTA4956.3100742E-98.788052145
GAGCGTA12651.1230532E-68.597008145
CCGATCT10356.5962365E-47.70521267
GTGTACG18456.1631E-77.0733104145
CTTCCGA15852.7452445E-56.86101774
AGAGCGC38550.06.7705617145
TCGGAAA26503.747118E-96.566168145
AGTGTAG682100.06.1330194145
GTGTAGA662850.06.070498145
CGGTGGT415650.06.070131145
CGCCGTA291550.06.0428224145
TAGATCT582600.06.035564145
TCTCGGT460000.05.9734874145
TCGTGTA1120500.05.965759145
TGTAGGG1034800.05.9553747145
GTCGCCG331150.05.7799726145
Produced by FastQC (version 0.11.5)