FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005689014

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005689014
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6895266
Sequences flagged as poor quality0
Sequence length151
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTCGG20700.031.87377145
CGCCGTA44000.014.830379145
GCCGTAT41650.013.9263525145
GATCTCG190050.012.627649145
TCTCGGT150900.012.348275145
TGGTCGC79700.011.826261145
CGGTGGT120750.011.708732145
AGTGTAG314800.011.654085145
GTGGTCG91600.011.556325145
TAGATCT235150.011.531585145
TCGCCGT62450.011.377741145
CGTATCA37150.011.319613145
AGATCTC228600.011.069082145
GAGTGTA333500.011.044098145
GGTCGCC86450.010.902868145
GTGTAGA299350.010.778123145
GTAGATC320750.010.714553145
GTCGCCG70100.010.446378145
CTCGGTG167800.010.326862145
CCGTATC43200.010.2378435145
Produced by FastQC (version 0.11.5)