FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005689024

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005689024
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6014052
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTCGG10800.030.875717145
GAGTGTA142300.012.277081145
AGTGTAG133650.011.986883145
GTGTAGG347150.011.589342145
CGGTGGT50100.011.575379145
CGTGTAG360100.011.534917145
TCGTGTA389600.011.44298145
AGAGTGT174100.011.2837515145
GTGTAGA131600.011.182094145
TGTAGGG325150.010.812869145
TAGGGAA285500.010.791102145
GTCGTGT417250.010.702059145
GTAGGGA304400.010.502118145
CGTCGTG457200.010.274288145
AGGGAAA325350.010.2046385145
AAAGAGT217950.010.14439145
GTAGATC150250.010.131827145
AGCGTCG517050.09.743955145
AAGAGTG219450.09.711688145
GATCTCG79750.09.635142145
Produced by FastQC (version 0.11.5)