FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005689030

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005689030
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6176925
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTCGG16500.025.045322145
TCGCGTA2155.0603924E-420.23245145
TGGTCGC47400.012.083269145
AGTGTAG183650.011.882593145
GTGGTCG54100.011.7929125145
TAGATCT142400.011.557171145
AGAGCGG36500.011.520487145
GTGTAGA175000.010.854227145
CCGTATC27900.010.654065145
GCCGTAT26600.010.6296425145
GATCTCG114200.010.538473145
TGTAGAT192300.010.330158145
AGAGTGT227700.010.28431145
TCTCGGT90400.010.185233145
GAGTGTA194650.010.093704145
CGTATCA25200.010.06939145
GTGTAGG424450.010.043532145
TAGGGAA357750.09.990862145
CGTGTAG438950.09.860409145
TCGTGTA463400.09.762571145
Produced by FastQC (version 0.11.5)