FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005689033

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005689033
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6453321
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG34200.012.9301605145
CTCGTAT52900.012.607597145
TCGTATG46950.012.043679145
ATCTCGT60900.011.546613145
CGTATGC42150.010.835355145
TCTCGTA56200.010.835355145
ACACATT224100.010.642758145
CATTGGC171700.010.639726145
ACATTGG180300.010.092022145
ATTGGCA156900.09.841399145
GTCACAC250750.09.7718315145
CACACAT248700.09.735783145
GTATGCC54500.09.710163145
AGTCACA305050.09.553339145
TCCAGTC354650.09.545905145
CACATTG202550.09.305533145
TGGCATC156600.09.304746145
CCAGTCA334350.09.236521145
AACTCCA440300.09.220182145
TCACACA266700.09.078699145
Produced by FastQC (version 0.11.5)