FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005689036

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005689036
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7372455
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTCGG17400.023.751524145
CGCCGTA28150.014.423661145
GCCGTAT28350.012.787416145
TCTCGGT107150.011.5033045145
AGTGTAG217850.011.049596145
CCGTATC28750.010.844173145
GTGTAGA208100.010.731108145
TGGTCGC53500.010.706295145
AGAGTGT272200.010.441522145
GAGTGTA229500.010.267544145
GATCTCG129300.010.26168145
GTCGCCG50850.010.12356145
TAGATCT170300.010.090197145
TGTAGGG449400.09.938333145
CGTGTAG485750.09.881231145
GGTCGCC66350.09.834843145
TCGCCGT44550.09.764936145
CGTATCA27550.09.737467145
TGTAGAT230050.09.675693145
GTAGATC224900.09.671586145
Produced by FastQC (version 0.11.5)