FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005689038

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005689038
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9165557
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTCGG27900.033.52171145
TGGTCGC140600.013.303779145
CGCCGTA80700.013.296239145
GCCGTAT77000.013.181898145
CCGTATC74300.012.977871145
GTCGCCG127500.012.111837145
TCTCGGT258100.012.022545145
CGGTGGT206000.011.578953145
TCGCCGT111650.011.493576145
AGAGCGG58700.011.362931145
GGTCGCC150800.011.346223145
TCGGTGG247650.010.890434145
GTGGTCG158400.010.801832145
TAGATCT391850.010.5646925145
CGTATCA67300.010.55727145
GATCTCG325350.010.339697145
GAGTGTA546050.010.130544145
AGTGTAG519700.010.128019145
AGATCTC377650.010.098036145
TGTAGAT511850.09.886745145
Produced by FastQC (version 0.11.5)