FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005689045

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005689045
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6980897
Sequences flagged as poor quality0
Sequence length151
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAAGTA80300.015.258129145
CACCCGA132950.014.178006145
ACCCGAA111750.014.013168145
CCGAAGT99250.013.586656145
TCACCCG152250.012.809291145
ATCTCGT37500.012.56644145
CTCGTAT32800.012.156793145
CGTATGC24350.011.611699145
CCCGAAG121850.011.423675145
CGTCTGA490600.011.068389145
CCAGTCA269950.010.957386145
TCTCGTA36400.010.7553145
AACTCCA377300.010.549107145
TCGTATG29300.010.392303145
TCCAGTC290900.010.267948145
ACGTCTG532350.010.132248145
TGAACTC433150.09.975578145
GTCACCC232500.09.884768145
GTAATCT78550.09.875697145
TCTGAAC472300.09.854786145
Produced by FastQC (version 0.11.5)