FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005689066

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005689066
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8165172
Sequences flagged as poor quality0
Sequence length151
%GC51

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTCGG28200.028.536915145
TTAGGCA40300.018.9116041
TAGGCAT49750.012.0957132
GTGTAGA294050.011.218204145
TGGTCGC86100.011.114858145
TAGATCT226200.010.929364145
AGTGTAG305900.010.878433145
CCGTATC51200.010.761599145
CGCCGTA51550.010.688532145
GAGTGTA330900.010.538564145
GATCTCG198850.010.317963145
GTAGATC301600.010.144116145
TCGCGTA5056.625851E-810.049391145
AGATCTC237600.09.916766145
TCCGATC11703.4402547E-89.9139326
TGTAGAT296600.09.8506975145
AGAGTGT385150.09.750636145
TCTCGGT163750.09.607525145
CCGATCT15302.7830538E-109.4767867
TAGGGAA583100.09.387221145
Produced by FastQC (version 0.11.5)