FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005689068

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005689068
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8598826
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTCGG15000.019.816433145
CGCCGTA34550.015.318198145
GCCGTAT36000.011.076258145
GAGTGTA259150.010.658759145
AGTGTAG242150.010.508858145
CCGTATC36300.010.385552145
TAGGGAA461750.010.111412145
CGTGTAG563250.010.065571145
GTAGATC259300.010.037482145
TCTCGGT127150.09.978253145
TGGTCGC65700.09.93139145
CGTATCA33600.09.925478145
GTGGTCG74350.09.848572145
AGAGTGT305500.09.801029145
GTGTAGG542050.09.763743145
GTCGCCG65350.09.7627145
GTCGTGT634550.09.597239145
CGGTGGT102700.09.530072145
TCGTGTA595350.09.376726145
GTGTAGA236850.09.335968145
Produced by FastQC (version 0.11.5)