FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005689070

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005689070
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6237131
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTCGG14100.034.449776145
CGCCGTA27500.012.127069145
CGTGTAG401000.010.377631145
GATCTCG115100.010.2039995145
GAGTGTA187750.010.117007145
AGTGTAG179600.010.051334145
CGTATCA27500.010.018014145
GTGTAGA174450.010.015596145
TGTAGGG369100.09.762096145
TCTCGGT90900.09.7303095145
GTGGTCG57450.09.716983145
GTAGGGA344550.09.637047145
GTAGATC184750.09.614182145
TCGTGTA426350.09.590551145
AGAGTGT219750.09.534539145
GGGAAAG332700.09.282982145
AGATCTC138400.09.271882145
GTCGCCG48800.09.210911145
TGGTCGC49800.09.171534145
CGTCGTG495050.09.094384145
Produced by FastQC (version 0.11.5)