FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005689076

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005689076
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6312473
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTCGG17150.027.479774145
GTGGTCG93500.015.586492145
CGCCGTA44250.014.746643145
CCGTATC42600.014.12643145
TGGTCGC80750.014.096817145
GCCGTAT43000.013.320562145
TCGGTGG146050.011.566934145
GATCTCG194000.011.510996145
AGAGCGG41100.011.290212145
GGTCGCC88050.011.198851145
TCTCGGT156900.011.182948145
TAGATCT243400.011.170551145
TCGCGTA3258.18909E-911.154512145
TCGCCGT61750.011.154511145
CTCGGTG166700.011.090943145
GTGTAGA304350.010.672561145
GTCGCCG70750.010.65788145
AGTGTAG316100.010.551087145
CGGTGGT123600.010.500221145
AGAGTGT378350.010.386506145
Produced by FastQC (version 0.11.5)