FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005689080

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005689080
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6509605
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATTCGT9850.041.2129334
CGATTCG2502.4501787E-929.003373
AGATTCG18800.026.6121353
ATTCGTG10900.025.9370085
ATTCGCG1952.6191864E-422.3048025
GCGTCGG17150.021.984892145
CGCCGTA40900.014.182468145
TGGTCGC68700.013.615015145
GTGGTCG79900.012.523262145
GCCGTAT39000.012.270564145
TCAGATT80300.012.24211
CCGTATC39400.012.145988145
GATCTCG168550.012.08823145
TCCGATC10303.7107384E-1011.9644696
CCGATCT12951.0913936E-1111.1953747
TCGCCGT55200.011.165161145
CGTATCA36700.011.063872145
CGGTGGT110300.010.978073145
TCTCGGT139100.010.790173145
GTCGCCG64600.010.662923145
Produced by FastQC (version 0.11.5)