FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005689084

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005689084
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11311114
Sequences flagged as poor quality0
Sequence length151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTCGG23150.025.051521145
CGCCGTA52850.014.265384145
AGTGTAG385650.011.297341145
GTAGATC413900.011.139273145
GAGTGTA410050.011.137787145
TCTCGGT190250.011.126365145
GCCGTAT53400.010.996105145
CCGTATC54250.010.957443145
GATCTCG247900.010.907556145
TGGTCGC98800.010.859252145
AGAGTGT474550.010.586352145
GTCGCCG93250.010.572682145
AAGAGTG569650.010.422477145
GTGGTCG113400.010.35612145
TCGCCGT83750.010.300475145
CGGTGGT156300.010.296488145
TAGATCT296100.010.25819145
TGTAGAT401350.010.223234145
GTGTAGA375600.010.171388145
AGATCTC292900.09.627763145
Produced by FastQC (version 0.11.5)