FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005689089

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005689089
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7592184
Sequences flagged as poor quality0
Sequence length151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC47600.014.7735615145
TCGTATG55850.013.499902145
ACGAGCT206800.012.690492145
ATCTCGT71700.012.63896145
TATGCCG39400.012.512183145
CACGAGC224400.012.308994145
GTCACGA256800.011.913459145
CTCGTAT61950.011.702513145
CGAGCTG216950.011.328188145
TCTCGTA67650.011.2523155145
TCACGAG246500.011.087787145
AGTCACG289400.010.997309145
CCAGTCA401100.09.651815145
TCCAGTC427300.09.569002145
TGAACTC605900.09.344811145
AACTCCA530900.09.176498145
CGTCTGA679800.09.171444145
CAGTCAC392350.09.017095145
CTCCAGT484500.08.858258145
ACGTCTG730250.08.795946145
Produced by FastQC (version 0.11.5)