FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005689094

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005689094
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8650978
Sequences flagged as poor quality0
Sequence length151
%GC51

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTCGG21050.022.38695145
CGCCGTA36900.015.914475145
AGTGTAG315600.012.289959145
TAGATCT225800.011.944078145
AGATCTC234600.011.928696145
GATCTCG196450.011.920217145
GAGTGTA349600.011.882748145
TCTCGGT154650.011.860535145
TGTAGAT306850.011.67171145
AGAGTGT395150.011.650523145
GTGTAGA299300.011.554345145
GTGGTCG87800.011.147384145
GTAGATC318300.010.796311145
AAAGAGT469450.010.748642145
TGGTCGC73100.010.711247145
CGTATCA32100.010.615159145
TAGGGAA647600.010.400221145
AAGAGTG482050.010.317291145
TCGCCGT64800.010.293108145
CGGTGGT122950.010.2012145
Produced by FastQC (version 0.11.5)