FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005689096

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005689096
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7611063
Sequences flagged as poor quality0
Sequence length151
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTCGG20900.035.728283145
TCGCGTA3403.2514246E-517.058165145
CGCCGTA32250.014.162244145
CCGTATC32650.013.322609145
TGGTCGC63450.012.796983145
GCCGTAT32500.012.714894145
GTGGTCG77150.012.497897145
AGAGCGG47300.012.108411145
GATCTCG166700.011.655218145
AGTGTAG252450.011.257239145
GAGTGTA271300.010.982805145
TAGATCT193800.010.661352145
GTCGCCG62800.010.620608145
TGTAGAT264750.010.597325145
GTGTAGA245400.010.310319145
TCGCCGT53950.010.212766145
CGTATCA28450.010.192928145
GTAGATC261600.010.142957145
AAGAGTG369200.010.093055145
TCTCGGT125250.010.013585145
Produced by FastQC (version 0.11.5)