FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005689100

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005689100
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7594907
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTCGG14350.023.240366145
CGCCGTA29050.013.975872145
GCCGTAT29250.013.384584145
GTGGTCG66900.011.920751145
AGTGTAG247000.011.68216145
GAGTGTA261400.011.621052145
TCTCGGT116750.011.612393145
GATCTCG147150.011.430488145
TGGTCGC55850.011.293618145
TAGATCT186500.010.84582145
CCGTATC30250.010.785099145
CGGTGGT93800.010.743579145
GTAGATC255800.010.203261145
AGATCTC182000.010.157944145
TGTAGAT257100.010.151669145
GTGTAGA238250.010.011519145
GTAGGGA515250.09.9902725145
GTGTAGG577750.09.900886145
AGAGTGT300500.09.891825145
TCGTGTA636000.09.826236145
Produced by FastQC (version 0.11.5)