FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005689103

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005689103
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6114078
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG29150.014.176089145
AGTCACC191500.011.470809145
CCAGTCA238400.010.400161145
CAGTCAC238050.010.384997145
CTCGTAT31550.010.340305145
AACTCCA329550.010.229439145
TATGCCG19900.010.200586145
GTCACCA197650.010.16022145
TGAACTC379250.010.150539145
CGTCTGA431500.010.030294145
TCTCGTA35500.010.006631145
ATCTCGT36050.09.853964145
ACGTCTG467350.09.834837145
ACACGTC526350.09.792986145
TCTGAAC417500.09.654693145
CACACGT568750.09.50906145
GTCTGAA453200.09.48604145
CTCCAGT296350.09.442838145
GAACTCC354700.09.381488145
CACGTCT507100.09.206879145
Produced by FastQC (version 0.11.5)