FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005689106

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005689106
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5589905
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTCGG14450.037.625557145
CGCCGTA78100.015.129553145
GGTCGCC124150.012.086849145
GTGGTCG138800.011.907892145
GTCGCCG110500.011.743032145
TCGCCGT99100.011.630891145
TGGTCGC123750.011.422967145
CGTATCA65200.011.340758145
TCGGTGG205200.011.304781145
GCCGTAT72950.011.22904145
TCTCGGT221850.011.207899145
GATCTCG266750.011.169325145
CGGTGGT172550.010.965163145
AGAGCGG31150.010.472346145
CCGTATC71100.010.297725145
TAGATCT322000.010.175882145
GTGTAGA397900.010.147774145
TGTAGAT402450.010.105095145
AGTGTAG417550.010.017443145
AGATCTC304450.09.8100395145
Produced by FastQC (version 0.11.5)