FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005689117

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005689117
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6684512
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTCTG197000.011.408258145
ACACGTC220050.010.608607145
CACACGT246050.010.430468145
CGTCTGA177500.09.761654145
CGCGATC10407.344497E-79.7590783
GCACACG273550.09.699922145
CACGTCT217800.09.45332145
TAGATCG170100.09.206049145
CGCGCGT9552.5782136E-59.1720851
GTCTGAA218300.08.6346245145
TCTGAAC204950.08.524973145
GATCGGA473050.08.367765145
CTGAACT210750.08.3247595145
AGATCGG505350.08.292003145
TCGGAAG434750.08.287827145
AGAGCAC357500.08.152182145
TATGCCG12550.08.087386145
ATCGGAA452850.08.052625145
AGCACAC328400.07.903214145
ACAGTAC73500.07.8908997145
Produced by FastQC (version 0.11.5)