FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005689128

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005689128
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8857927
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTCGG24500.034.921425145
CGCCGTA47450.013.905326145
GTGGTCG101500.013.429746145
AGATCTC273850.011.914499145
GTGTAGA370750.011.91001145
TGGTCGC85200.011.829087145
GATCTCG225500.011.607446145
TCGGTGG176350.011.594436145
CGTATCA43500.011.501006145
GGTCGCC100250.011.427434145
TCTCGGT184550.011.314997145
TAGATCT285800.011.03578145
GTAGATC401250.011.004702145
AGTGTAG383300.010.952562145
CGGTGGT137550.010.911532145
GCCGTAT48050.010.5628395145
AGAGTGT466200.010.544681145
TGTAGAT388100.010.368725145
GAGTGTA410300.010.320181145
CTCGGTG208050.010.31573145
Produced by FastQC (version 0.11.5)