FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005689129

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005689129
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6878105
Sequences flagged as poor quality0
Sequence length151
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT62900.014.52296145
ATCTCGT76200.014.461851145
CGTATGC48550.014.036985145
TCTCGTA69300.013.600203145
TCGTATG57350.013.400089145
GTCACTA171600.013.350732145
TATGCCG40450.012.725506145
TCACTAA172400.012.027185145
CTAATCT120450.011.556589145
ACTAATC118300.011.460198145
CAGTCAC587500.011.390141145
CACTAAT140300.011.213399145
GTCACAG348200.010.889505145
GTATGCC60100.010.856835145
CCAGTCA422950.010.5591145
CACAGTC302800.010.22368145
TCCAGTC453300.010.092038145
AGTCACA369250.09.817135145
TCACAGT340100.09.741926145
AGTCACT233400.09.722517145
Produced by FastQC (version 0.11.5)