FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005689132

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005689132
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6237785
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTCGG15250.029.000134145
GCCGTAT43250.015.92493145
CGCCGTA43950.013.361836145
TGGTCGC76550.012.78582145
CGTATCA38500.012.42863145
TCTCGGT155200.012.285817145
GGTCGCC83550.012.148471145
GTCGCCG70550.012.023443145
GTGGTCG89400.012.002294145
TCGGTGG145400.011.817455145
CGGTGGT120950.011.808654145
CCGTATC41800.011.273975145
TCGCCGT61350.011.108446145
GTGTAGA305100.011.002181145
GATCTCG190200.010.901732145
AGTGTAG318300.010.750914145
CTCGGTG165750.010.6727495145
GAGTGTA336350.010.561964145
GTAGATC318950.010.478965145
AGATCTC227350.010.332136145
Produced by FastQC (version 0.11.5)