FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005689143

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005689143
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7428600
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGCGAC101150.015.912062145
CATAGCG138300.014.41617145
ATAGCGA117750.013.97669145
AGCGACA105500.013.95028145
CGACATC82500.013.4454975145
CGTATGC31850.013.202553145
CTCGTAT41750.013.023993145
TCGTATG35750.012.573466145
ATCTCGT50800.012.416378145
ACATAGC179650.012.0262145
GCGACAT75800.011.955842145
TCACATA223350.011.52343145
CATCTCG73450.011.055175145
GTCACAT244650.010.638701145
TCTCGTA48100.010.400241145
AACTCCA441250.010.318446145
CACATAG203200.010.311303145
CCAGTCA326350.010.2857685145
CGTCTGA579950.010.038397145
TGAACTC508250.09.985275145
Produced by FastQC (version 0.11.5)