FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005689152

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005689152
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6949770
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTCGG12100.024.5655145
CGCCGTA14700.014.7955475145
AGTGTAG155500.012.028638145
GATCTCG86600.011.97141145
GAGTGTA162400.011.472927145
GTCGCCG32050.011.310169145
GTGTAGA150000.010.536404145
TCTCGGT70950.010.524755145
AGAGTGT200550.010.374958145
CGTGTAG367600.010.275178145
CGATCGG5700.00256048510.175184145
GTAGATC161100.09.990439145
CGGTGGT57450.09.96929145
TAGGGAA306050.09.949105145
AGATCTC121850.09.936149145
AGAGCGG35800.09.922936145
GTGTAGG360250.09.90121145
TGTAGGG345250.09.869413145
TGGTCGC32100.09.711595145
TCGTGTA389200.09.704922145
Produced by FastQC (version 0.11.5)