FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005689161

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005689161
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8416390
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGCTTA95350.016.575562145
CGCTTAA86050.016.092182145
ATCTCGT50600.013.754764145
ACAACGC143400.013.347109145
AACGCTT110100.013.235525145
CACAACG155200.012.612596145
CAACGCT121050.012.517394145
TCACAAC202650.012.056347145
CGTATGC31850.011.608956145
TCGTATG37150.011.513984145
CGTCTGA562600.010.863263145
GTCACAA238600.010.847514145
TCTCGTA45600.010.811265145
CTCGTAT41850.010.7406025145
ACACGTC690800.010.285045145
TATGCCG26850.010.260576145
TCTGAAC546600.010.213005145
TCCAGTC347700.09.904244145
AACTCCA434800.09.871078145
ACGTCTG610000.09.638811145
Produced by FastQC (version 0.11.5)