FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005689162

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005689162
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8416390
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTCGG19250.038.790737145
TGGTCGC54650.013.398395145
GAGTGTA245350.011.908047145
CGCCGTA26650.011.697493145
TCTCGGT109600.011.5757475145
TCGCGTA3151.8686645E-411.5075145
AGTGTAG234050.011.274941145
GTGGTCG63700.011.267234145
GTGTAGA230750.010.964915145
TAGATCT184350.010.735964145
GCCGTAT30050.010.615238145
GTAGGGA495850.010.483116145
GTGTAGG567350.010.36314145
TGTAGAT253600.010.26282145
GATCTCG143450.010.259283145
TAGGGAA464050.010.107903145
AGAGCGG47350.010.105214145
AGAGTGT293100.010.042267145
TGTAGGG534050.09.97762145
GTCGTGT661350.09.865809145
Produced by FastQC (version 0.11.5)