FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005689164

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005689164
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6458371
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTCGG12850.030.462975145
CGCCGTA28500.013.480709145
TGGTCGC51600.012.222253145
GCCGTAT30600.011.84487145
AGTGTAG204050.011.403814145
AGATCTC158000.010.7359495145
GTCGCCG48100.010.398859145
GTGGTCG61250.010.296624145
TCGCCGT45800.010.287968145
GATCTCG133350.010.219897145
CCGTATC31350.010.17412145
GTGTAGA196050.09.983403145
AGAGTGT251000.09.963847145
GAGTGTA216100.09.828666145
TGTAGGG434150.09.684337145
TCTCGGT103050.09.637276145
TAGATCT163400.09.582601145
GTAGGGA407050.09.509884145
CGTGTAG466550.09.415776145
TCGGTGG102750.09.383211145
Produced by FastQC (version 0.11.5)