FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005689166

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005689166
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5600664
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTCGG15500.032.74324145
CGCCGTA48900.015.86464145
TCGCGTA2900.003755343615.000598145
GCCGTAT45400.014.37282145
CCGTATC43850.013.062095145
TGGTCGC82900.012.681446145
GGTCGCC88050.012.6807995145
GTCGCCG73950.012.451476145
GTGGTCG95650.012.052232145
TCTCGGT160050.011.778038145
CGTATCA40050.011.767011145
CGGTGGT127200.011.684821145
TCGGTGG151800.011.224096145
GGTGGTC141700.010.847292145
GTGTAGA299750.010.691001145
AGATCTC230500.010.56875145
GATCTCG192550.010.430174145
GAGTGTA328750.010.211053145
TAGATCT238400.010.188115145
TCGCCGT65700.010.152612145
Produced by FastQC (version 0.11.5)