FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005689168

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005689168
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7358794
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTCGG15250.033.753258145
CGCCGTA30600.011.372265145
GCCGTAT31850.010.69832145
GATCTCG122700.010.044574145
TGGTCGC54350.010.00435145
CGTGTAG403350.09.759891145
GTGTAGG394300.09.708102145
GAGTGTA191600.09.686605145
GTCGCCG51250.09.477813145
GTAGGGA355150.09.451405145
TGTAGGG379450.09.438426145
GTGGTCG61300.09.34316145
TCGTGTA427150.09.317924145
TAGGGAA336800.09.212954145
TCTCGGT95800.09.081193145
GTGTAGA186150.09.035499145
GTAGATC196500.09.00232145
GAGCGTC586650.08.934833145
GTCGTGT453600.08.934411145
CGATCGG7450.00233763348.758171145
Produced by FastQC (version 0.11.5)