FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005689169

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005689169
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14191378
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG96500.011.644542145
TCTCGTA113250.011.3306055145
CGTATGC84650.010.534069145
CTCGTAT106850.010.516598145
ATCTCGT123750.010.486387145
GTCACAC488950.010.275118145
TCCAGTC694950.09.972921145
CCAGTCA649800.09.628289145
CGTCTGA1109800.09.374005145
AACTCCA862600.09.286902145
TATGCCG71450.09.233306145
TCACACA520950.09.18472145
GAACTCC934900.09.119273145
CACGTCT1283650.09.064554145
TCTGAAC1074600.08.938963145
CAGTCAC638500.08.935768145
CACACGT1446850.08.858839145
ACGTCTG1185950.08.857681145
AGTCACA588750.08.853521145
ACACGTC1334550.08.767721145
Produced by FastQC (version 0.11.5)