FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005689175

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005689175
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7848310
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGGAACAAATCTCGTAT177290.22589576609486628TruSeq Adapter, Index 10 (97% over 36bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCTCGT98650.010.361568145
CGTATGC76900.09.238538145
TATGCCG66500.09.20065645-49
CTCGTAT87350.09.129212145
CTGAACT630500.08.86488145
AACTCCA525750.08.852364145
CCAGTCA400950.08.660616145
CAGTCAC384050.08.607571145
GAACTCC560200.08.527972145
TCACTGG333150.08.442973145
GTCTGAA688500.08.423441145
AGTCACT349600.08.356745145
GTCACTG348450.08.280301145
TCTCGTA92300.08.246907145
TGAACTC597650.08.21193145
GCCGTCT73950.08.17571750-54
ACGTCTG705250.08.171985145
TCCAGTC427950.08.148085145
ACACGTC780850.08.123514145
TCTGAAC647300.08.063626145
Produced by FastQC (version 0.11.5)