FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005689177

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005689177
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7654302
Sequences flagged as poor quality0
Sequence length151
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCTCGT132750.014.635832145
CTCGTAT111150.014.479736145
TCGTATG98550.014.197695145
CGTATGC88500.013.434382145
GTTAGCA263550.013.09366145
GAGTTAG316250.012.883176145
CGAGTTA326600.012.874458145
ACGAGTT366150.012.552995145
TCTCGTA121900.012.429674145
GTCACGA468850.011.952609145
TCACGAG442000.011.940602145
AGTTAGC283000.011.937589145
CATCTCG168150.011.899517145
TATGCCG71400.011.778146145
CACGAGT413200.011.1938145
TAGCATC223800.010.981377145
GCATCTC219900.010.945359145
GTATGCC99150.010.748349145
TTAGCAT243250.010.371547145
AGTCACG515050.010.345586145
Produced by FastQC (version 0.11.5)