FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005689180

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005689180
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6644265
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTCGG13900.037.030453145
GCCGTAT30700.015.349366145
CGCCGTA30000.014.982555145
TGGTCGC58750.014.190754145
GTGGTCG71850.014.125918145
TAGATCT207000.012.818174145
TCTCGGT134300.012.6854925145
AGTGTAG280250.012.468576145
GATCTCG166250.012.2535145
AGATCTC196750.012.196318145
TCGGTGG121100.011.972954145
GTGTAGA270650.011.892972145
CGGTGGT99100.011.85105145
GTCGCCG53650.011.756146145
CGTATCA28600.011.6602335145
TCGCCGT46600.011.201136145
GAGTGTA305950.010.876213145
CCGTATC30900.010.79232145
GGTCGCC69200.010.790625145
TGTAGAT284000.010.51706145
Produced by FastQC (version 0.11.5)