FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005689182

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005689182
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6266233
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTCGG14250.016.789278145
CGCCGTA24200.014.080413145
TGGTCGC48650.012.5178385145
GATCTCG129600.011.97131145
GAGTGTA219550.011.326445145
TAGATCT159500.011.318049145
GCCGTAT26300.011.302149145
GTGGTCG57800.011.163364145
AGTGTAG208250.011.001072145
CGGTGGT80550.010.800618145
GTGTAGA200100.010.724511145
CCGTATC28550.010.665375145
GTCGCCG47250.010.43374145
TCTCGGT99500.010.4194765145
TCGCCGT41850.010.394143145
TGTAGAT218450.010.188711145
TAGGGAA410250.010.161367145
GTGTAGG483700.010.102218145
CGTATCA25400.09.990041145
CGTGTAG503300.09.939282145
Produced by FastQC (version 0.11.5)