FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005689184

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005689184
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7752003
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTCGG12700.018.838243145
CGCCGTA17400.012.916435145
GAGTGTA167400.010.957091145
GTCGTGT429500.010.786185145
AGTGTAG158900.010.767585145
GCGTCGT502750.010.541331145
GTGTAGG369800.010.488588145
TAGGGAA311050.010.488479145
GTAGGGA329000.010.445101145
CGTGTAG378650.010.358325145
CGTCGTG472200.010.317476145
TCTCGGT75200.010.3156395145
TCGTGTA401450.010.203453145
AGCGTCG542900.010.0688925145
TGTAGGG350950.09.936422145
TAGATCT125200.09.9019785145
GTGTAGA158350.09.79774145
GTAGATC172950.09.725179145
AGAGTGT204650.09.600365145
GAGCGTC583100.09.374724145
Produced by FastQC (version 0.11.5)