FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005689197

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005689197
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7231591
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC28300.011.271944145
CGTCTGA376800.010.582405145
ATCTCGT43200.010.572784145
TCGTATG31750.010.275462145
ACACGTC458200.010.2530365145
ATTGAGG126650.09.903153145
TCCAGTC244750.09.745537145
CACACGT500100.09.712936145
ACGTCTG407650.09.585922145
GTCTGAA404900.09.382446145
TATGCCG22450.09.36514145
GTCACAT171550.09.212943145
TGAACTC343850.09.150679145
CTGAACT369700.09.020715145
TCTGAAC379750.08.915623145
GAACTCC328900.08.883288145
CACGTCT445900.08.812404145
AGCACAC620800.08.641967145
GCACACG544250.08.591992145
CGGAAGA851600.08.470714145
Produced by FastQC (version 0.11.5)