FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005689200

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005689200
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8820277
Sequences flagged as poor quality0
Sequence length151
%GC50

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTCGG25550.033.199726145
GTGTCCT107750.020.4820041
CCTTGTG101150.019.279425
GTCCTTG126000.016.8600483
TGTCCTT159750.014.29655552
TCCTTGT136150.013.8976944
TTGTGAC66900.013.6541397
CTTGTGA94300.012.607746
GCCGTAT51450.012.259519145
GAGTGTA375050.011.269871145
CGTATCA46350.011.262176145
TGGTCGC92800.011.015664145
TCTCGGT181750.011.009668145
GTGGTCG107500.010.925621145
GATCTCG216250.010.82894145
CGCCGTA52000.010.735616145
AGTGTAG339800.010.646742145
GTGTAGA327950.010.633518145
TAGATCT259600.010.444953145
AGATCTC257850.09.925379145
Produced by FastQC (version 0.11.5)