FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005689205

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005689205
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6630671
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG51900.013.689555145
CGTATGC44850.012.931776145
CTCGTAT57750.012.930517145
CTTCGAA130750.012.364991145
TCGAATC98000.012.206426145
CACTTCG150650.011.98287145
ATCTCGT68650.011.511094145
TTCGAAT106750.011.409643145
TATGCCG36600.011.2907915145
ACTTCGA142600.011.0832615145
TCTCGTA63200.010.8977585145
CGAATCT90950.010.601799145
TCCAGTC368800.09.7110615145
AGTCACC288550.09.572702145
CAGTCAC340900.09.527559145
AACTCCA459800.09.208196145
GTCACCA290200.09.093575145
CCAGTCA345350.09.0899145
GAACTCC502750.08.969515145
TCACCAC288750.08.913269145
Produced by FastQC (version 0.11.5)