FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005689206

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005689206
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6630671
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTCGG18450.034.186012145
CGCCGTA41100.013.582335145
AGAGCGG43200.013.425542145
CCGTATC40450.011.291396145
TCGCCGT57900.011.269108145
GAGTGTA281800.011.216854145
CGTATCA37400.011.049149145
GCCGTAT40450.010.75371145
GTCGCCG67500.010.740434145
TGGTCGC73900.010.693198145
TCTCGGT136350.010.527752145
GTGGTCG82200.010.495441145
GTGTAGA258050.010.394976145
GATCTCG175350.010.336174145
AGTGTAG268200.010.217828145
TCGGTGG133600.09.876214145
TAGATCT210700.09.875133145
AGATCTC203000.09.856861145
AGAGTGT323850.09.71564145
TGTAGAT272950.09.588478145
Produced by FastQC (version 0.11.5)